Staphylococcus Aureus
  Created by Peter Acquafredda

Identification and Diagnosis

Gram stain of Staphylococcus aureus cells.
Staphylococcus aureus is identified using both biochemical and enzyme tests:

1.     Gram Stain (Shape/Configuration): 

Staphylococcus aureus is a Gram-positive bacterium, showing typical cocci of spherical bacterium in clusters.

2.     Culture (mannitol salt agar):

Staphylococcus aureus produces large yellow colonies.

3.     Coagulase Test:

Staphylococcus aureus is coagulase-positive (produces the enzyme coagulase which causes clot formation).

4.     Catalase Test:

Staphylococcus aureus is catalase-positive (produces the enzyme catalase which converts hydrogen peroxide to water and oxygen; bubbles observed).

Fact:Staphylococcus aureus can grow at temperatures of 15-45 °C and at salt concentrations as high as 15%.